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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, happen to be explained. Two added splice variants were described but comprehensive nucleotide sequence details is not still obtainable.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly contains the epitope acknowledged because of the antibody.
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This guide outlines further possible optimization of mobile and immunoassay parameters to be certain the very best outcomes are...
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This information outlines even further probable optimization of cellular and immunoassay parameters to make sure the very best effects are...
Antibodies sure to the blocking peptide now not bind towards the epitope around the focus on protein. By evaluating the staining through the blocked antibody versus the antibody by itself, one can see which staining is unique.
Goal Information and facts Cyclin E belongs to your hugely conserved cyclin family members, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation designs which lead to the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and features as a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S transition. This protein accumulates with the G1-S period boundary and is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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